Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CD43-mediated IFN-? production by CD8 + T cells promotes abdominal aortic aneurysm in mice

doi: 10.4049/jimmunol.1203228

Figure Lengend Snippet: (A) Sections of WT and CD43−/− aortas were stained for neutrophils (Gr-1+ cells), macrophages (Mac-3+ cells), T cells (CD3+ cells) and apoptotic cells (TUNEL+ cells). Scale bar, 100 μm. The number of Gr-1+ cells (B), Mac-3+ cells (C), CD3+ cells (D), and TUNEL+ cells (E) per aortic cross-section were enumerated. Values represent mean ± SEM; analysis was performed on 6–9 serial cross-sections per aorta, n = 4–5 aortas per genotype. *P < 0.0001.

Article Snippet: Neutrophils, macrophages, and CD3 + T cells were visualized with a biotinylated anti-Gr1 mAb (1:100 dilution, BD Biosciences), anti-Mac-3 mAb (1:200 dilution, Cedarlane Laboratories) and anti-CD3 mAb (1:100 dilution, BD Biosciences), respectively.

Techniques: Staining, TUNEL Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CD43-mediated IFN-? production by CD8 + T cells promotes abdominal aortic aneurysm in mice

doi: 10.4049/jimmunol.1203228

Figure Lengend Snippet: Mice were perfused with elastase on d0 and increase in AD on d14 was expressed as % (A) or mm (B). Values represent mean ± SEM. D14 elastin degradation (C) and SMC actin depletion (D) were graded on a scale of 1–4; Mac-3+ macrophages (E) were enumerated per aortic cross-section, n = 5 aortas per treatment. *P < 0.001 compared with WT.

Article Snippet: Neutrophils, macrophages, and CD3 + T cells were visualized with a biotinylated anti-Gr1 mAb (1:100 dilution, BD Biosciences), anti-Mac-3 mAb (1:200 dilution, Cedarlane Laboratories) and anti-CD3 mAb (1:100 dilution, BD Biosciences), respectively.

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CD43-mediated IFN-? production by CD8 + T cells promotes abdominal aortic aneurysm in mice

doi: 10.4049/jimmunol.1203228

Figure Lengend Snippet: (A) D14 aortic sections from WT and CD43−/− mice were stained for Mac-3, MMP-2 and MMP-9. L, lumen. Scale bar, 50 μm. Mac-3+ (B), MMP-2+ (C) and MMP-9+ (D) cells were enumerated in aortic cross sections following different treatment conditions. (E) In situ zymography with a fluorescein-labeled DQ gelatin substrate was used to detect the gelatinolytic activity of MMP on d14 aortic sections. (F) Images were analyzed by ImageJ software and gelatinase activity was normalized to the intensity of WT sections, which was set at 100%. Values represent mean ± SEM, n = 6–9 sections per aorta, 4–5 aortas per genotype/treatment. *P < 0.01, **P < 0.001 compared with WT.

Article Snippet: Neutrophils, macrophages, and CD3 + T cells were visualized with a biotinylated anti-Gr1 mAb (1:100 dilution, BD Biosciences), anti-Mac-3 mAb (1:200 dilution, Cedarlane Laboratories) and anti-CD3 mAb (1:100 dilution, BD Biosciences), respectively.

Techniques: Staining, In Situ, Zymography, Labeling, Activity Assay, Software

Journal: Cell Reports Medicine

Article Title: Mechanical confinement promotes heat resistance of hepatocellular carcinoma via SP1/IL4I1/AHR axis

doi: 10.1016/j.xcrm.2023.101128

Figure Lengend Snippet: 3D bioprinted GCL construct (A) The schematic representation of the 3D thermal ablation model using the mixture of GCL hydrogel and HCC cell lines. (B) A photograph of a 3D bioprinted model with a grid-like structure. (C and D) Live/dead staining of MHCC-97H and quantifications in 3D model for days 1 and 7 (three biological replicates). (E) Schematic diagram and infrared imaging of orthotopic HCC tumor during thermal ablation for 30 s in BALB/C nude mice. (F) Picrosirius red staining of fibrillar collagen in the different regions of the tumor tissue after thermal ablation for 30 s. (G and H) The images and volume of GCL structure after different heat treatments for 15 min (three biological replicates). (I and J) Collagen hybridizing peptide staining of fibrillar collagen in the different regions of tumor tissue after thermal ablation for 30 s (five biological replicates). (K and L) SEM images of fibrillar collagen (red arrows) and quantifications in GCL structure after different heat treatments for 15 min (three biological replicates). (M) The stiffness of 3D (five biological replicates) and animal (10 biological replicates) models detected by nano-indentation under different temperatures. Mean ± standard deviation. Statistical analysis, one-way ANOVA for (D), (H), (J), (L), and (M, left), two-tailed Student’s t test for (M, right). n.s., not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: A Picrosirius Red Staining Kit (ab150681, Abcam) was employed to reveal the fibrillar collagen of paraffinized tumor sections in the animal model of thermal ablation.

Techniques: Construct, Staining, Imaging, Standard Deviation, Two Tailed Test

Journal: Cell Reports Medicine

Article Title: Mechanical confinement promotes heat resistance of hepatocellular carcinoma via SP1/IL4I1/AHR axis

doi: 10.1016/j.xcrm.2023.101128

Figure Lengend Snippet:

Article Snippet: A Picrosirius Red Staining Kit (ab150681, Abcam) was employed to reveal the fibrillar collagen of paraffinized tumor sections in the animal model of thermal ablation.

Techniques: Recombinant, Bicinchoninic Acid Protein Assay, Imaging, Staining, Transfection, Software, Microscopy, Fluorescence, Liquid Chromatography, Mass Spectrometry

Journal: PLoS ONE

Article Title: CD105 + -mesenchymal stem cells migrate into osteoarthritis joint: An animal model

doi: 10.1371/journal.pone.0188072

Figure Lengend Snippet: A) Selective enrichment of CD105 + expressing cells. Fluorescence-activated cell sorting (FACS) analysis of the subpopulations of CD105 + pre-sorted (on the top) and post-sorted (on the bottom) from synovial membrane. 105 after was subpopulation of CD105 + post-sorted (on the left bottom) and 105- after was subpopulation of CD105 - post-sorted (on the right bottom). B) Characterization by flow cytometry analysis of the CD105 + -MSC population using MSCs markers, CD44, CD105, CD90 and haematopoietic markers CD45 and CD34. C) Flow cytometry of subpopulation of CD105 + -MSC labelled with oxacarbocyanine (DiO) and octadecyl (C18) indocarbocyanine (DiI) respectively. D) Characterization of GFP-CD105 + -MSCs used to validate previous data obtained using DiO- CD105 + -MSCs and DiI-CD105 + -MSCs in animal model. Fluorescence-activated cell sorting (FACS) analysis of the MSCs populations (on the left), GFP-MSCs (on the middle) and post-sorted for CD105 + (on the right) from synovial membrane. Representative image of GFP-CD105 + -MSCs in a plate before injection, bar represents 20 μm.

Article Snippet: The primary antibodies used were mouse anti-human CD34 (1:20 DakoCytomation, Barcelone, SP), FITC mouse anti-human CD45 (1:20), FITC mouse anti-human CD105 (1:100 from Serotec, Bavaria, GER), FITC mouse anti-human CD44 (1:100 from Serotec, Bavaria, GER) and PE-Cy5-conjugated mouse anti-human CD90 (1:20 from BD Pharmagen, Madrid, SP).

Techniques: Expressing, Fluorescence, FACS, Membrane, Flow Cytometry, Animal Model, Injection

Journal: PLoS ONE

Article Title: CD105 + -mesenchymal stem cells migrate into osteoarthritis joint: An animal model

doi: 10.1371/journal.pone.0188072

Figure Lengend Snippet: A) Co-localization of DiO-CD105 + -MSCs with antibodies against anti-CD105, anti-CD44 and anti-CD90 in crossed ligaments sections of left knee from animals injected with DiO-CD105 + -MSCs IV (magnification were 20x, 40x and 60x). Arrows point cells which co-localized for CD44 and CD90 antibodies. B) Histogram showed percentage of DiO positives signal normalized with DAPI signal from animals IA injected in front of animals IV injected. AnalySIS Image Processing was used. C) Quantitative analysis to determine levels of positive DiO fluorescence in front of DAPI signal was done by AnalySIS Image software from tissues where DiO-CD105 + -MSCs were found. D) Immunofluorescence analysis of sections from patella femoral grove and synovial membrane from the left knee at different magnifications (10x, 20x and 40x) from animals injected IA with DiO-CD105 + -MSCs. Arrows point the same group of cells which co-localized for CD105, CD44 and CD90 antibodies. E) Immunofluorescence analysis of sections from synovial membrane at 20x and 40x magnifications from right knee (up) and left knee (down). F) Histogram showed percentage of CD44, CD90 and CD105 positives signal normalized with DAPI signal from animals injected IA with DiO-CD105 + -MSCs. AnalySIS Image Processing was used. * P value less than 0.05 was considered statistically significant by Mann Whitney and Kruskall-Wallis.

Article Snippet: The primary antibodies used were mouse anti-human CD34 (1:20 DakoCytomation, Barcelone, SP), FITC mouse anti-human CD45 (1:20), FITC mouse anti-human CD105 (1:100 from Serotec, Bavaria, GER), FITC mouse anti-human CD44 (1:100 from Serotec, Bavaria, GER) and PE-Cy5-conjugated mouse anti-human CD90 (1:20 from BD Pharmagen, Madrid, SP).

Techniques: Injection, Fluorescence, Software, Immunofluorescence, Membrane, MANN-WHITNEY

Journal: Membranes

Article Title: Targeting the Structural Integrity of Extracellular Vesicles via Nano Electrospray Gas-Phase Electrophoretic Mobility Molecular Analysis (nES GEMMA)

doi: 10.3390/membranes12090872

Figure Lengend Snippet: Schematic drawing of the workflow for extracellular vesicle (EV) isolation and EV analysis performed in this research work. The following abbreviations are used: AA—ammonium acetate, nES GEMMA—nano electrospray gas-phase electrophoretic mobility molecular analyzer, nES DMA—nano electrospray differential mobility analyzer, NTA—nanoparticle tracking analysis, PBS—phosphate-buffered saline, RT—room temperature, SEC—size exclusion chromatography, US—ultrasonication.

Article Snippet: Subsequently, the single-charged, surface-dry particles were separated with a sheath flow of 8.0 L/min in a nano differential mobility analyzer (nDMA, model 3480C, TSI Inc.).

Techniques: Isolation, Gas Phase Electrophoretic Molecular Mobility Analysis, Size-exclusion Chromatography